Isolation of Density Enrichment Fraction of Adipose-Derived Stem Cells from Stromal Vascular Fraction by Gradient Centrifugation Method
نویسندگان
چکیده
منابع مشابه
Successful management of an equine carpal chip fracture by intra-articularly injected adipose-derived stromal vascular fraction after arthroscopic removal
Carpal chip fractures are common causes of lameness in racehorses. Due to disadvantages in surgical management, adjuvant treatment modalities are usually necessary. Adipose-derived stem cells (ADSCs) have the potential to differentiate into other cell types including bone and cartilage cells. Adipose-derived stromal vascular fraction (SVF) is produced during ADSCs isolation from adipose tissue....
متن کاملAdipose derived stromal vascular fraction improves early tendon healing: an experimental study in rabbits
Tendon never restores the complete biological and mechanical properties after healing. Bone marrow and recently adipose tissue have been used as the sources of mesenchymal stem cells, which have been proven to enhance tendon healing. Stromal vascular fraction (SVF), derived from adipose tissue by an enzymatic digestion, represents an alternative source of multipotent cells, which undergo differ...
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Discovering a cell type with the highest efficiency and the least side-effects is one of the priorities of scientists active in this field. Of all the cells, mesenchymal stem cells have been and still are of interest to the researchers for use in pre-clinical and clinical studies due to being easily accessible, pluripotency, secretion of numerous growth factors, low risk of immunological reject...
متن کاملMechanical versus enzymatic isolation of stromal vascular fraction cells from adipose tissue
Clinical use of adipose-derived stem cells (ASCs) for a variety of indications is rapidly expanding in medicine. Most commonly, ASCs are isolated at the point of care from lipoaspirate tissue as the stromal vascular fraction (SVF). The cells are immediately administered to the patient as an injection or used to enrich fat grafts. Isolation of ASCs from adipose tissue is a relatively simple proc...
متن کاملEffects of Uncultured Adipose Derived Stromal Vascular Fraction on Tendon Healing in Rabbits: A Histological and Immunohistochemical Study
Objective- To evaluate the potential effects of uncultured adipose derived stromal vascular fraction on tendon healing. Design- Prospective descriptive study. Animals- Twenty five adult male New Zealand white rabbits, weighing 2.5-3.0 kg were used. Five rabbits were used as donors of adipose tissue and the rest were divided into control and treatment groups. Procedures- The injury model was ...
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ژورنال
عنوان ژورنال: Endocrinology and Metabolism
سال: 2010
ISSN: 2093-596X
DOI: 10.3803/enm.2010.25.2.103